Journal: PLOS ONE

Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

doi: 10.1371/journal.pone.0301644

Figure Lengend Snippet: Gender-wise distribution of CHIKV cases based on ELISA and RT-PCR.

Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

Journal: PLOS ONE

Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

doi: 10.1371/journal.pone.0301644

Figure Lengend Snippet: Month-wise distribution of CHIKV cases based on ELISA (2A) and RT-PCR (2B).

Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

Journal: PLOS ONE

Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

doi: 10.1371/journal.pone.0301644

Figure Lengend Snippet: District-wise distribution of CHIKV cases based on ELISA (3A) and RT-PCR(3B). In this figure, we have analysed the district-wise prevalence rate of CHIKV by considering the total number of CHIKV sample tested and the samples showed positive results in both ELISA and RT-PCR assay. The samples showed ELISA positive results for CHIKV were not considered further for RT-PCR assay.

Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

Journal: PLOS ONE

Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

doi: 10.1371/journal.pone.0301644

Figure Lengend Snippet: Mean weight, blood sugar level and age of patients based on the RT-PCR and serological detection of CHIKV.

Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

Techniques: Enzyme-linked Immunosorbent Assay